3D-matrix scaffold

3D scaffolds and matrix gel culture systems are used to monitor and investigate their influence on growth, cell adhesion, proliferation and finally differentiation of neural progenitor cells. A clearly defined microenvironment is essential to support these parameters.
The use of hydrogel nano-scaffolds and the functionalization of the 3D-matrix lead to different growth patterns, influenced by a different assembling of the scaffolds as demonstrated by AFM microscopy. During differentiation, these environments support and enhance the number and percentage of cells in the hydrogel scaffold compared to 2D culture conditions.
Monitoring the proliferation and differentiation of neural progenitor cells with 3D matrix scaffolds, the aim of this project is to optimize conditions and to adopt these systems in order to find improved methods for standardized cell replacement therapies in neurodegenerative diseases.